: T-TC Y-JW. Analyzed the data: T-TC. Contributed reagents/materials/analysis tools: Y-QZ T-TC. Wrote the paper: T-TC Y-QZ.SAEW Degumming MethodpH 11.50 SAEW was used for degumming cocoons and calculation of the degumming rate. For each storage type of drySilk Degumming and the Physical PropertiesGonadal steroids modulate a myriad of male social behaviors that contribute to successful reprod
To get the 5' stop with the Ex4a(+)WT1 cDNA, the WT1-specific primers have been created upstream (F1, F2, F3, and F4) and downstream (F5, F6, and F7) with the important transcriptional start off internet site of WT1 that was registered in Database of Transcriptional Start off Web pages (Fig 2A). Complete RNA from K562 cells was reverse-transcribed with oligo(dT) primer and M-MLV reverse transcrip
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loop (E3) region near the channel pore [26]. The specificity of E3-targeting antibodies was tested by ELISA, Western blotting and functional assays, and fluorescence activated cell sorting (FACS) [9,26]. The procedure for Western blotting has been described previously [26]. Briefly, cells were lysed in RIPA buffer (Sigma-Aldrich, Poole, UK) and proteins were separated on 10 SDS-PAGE gel before tr
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